Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1C12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PP2C gamma Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PPM1G (NP_817092) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

59.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free

Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12)Azide and BSA Free [NBP2-73552]

Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12)Azide and BSA Free [NBP2-73552]

Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free [NBP2-73552] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G.
Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free [NBP2-73552]

Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free [NBP2-73552]

Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free [NBP2-73552] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1G.
Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12)Azide and BSA Free [NBP2-73552]

Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12)Azide and BSA Free [NBP2-73552]

Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free [NBP2-73552] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1G monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PP2C gamma

PPM1G is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Gene Symbol

PPM1G

Additional PP2C gamma Products

Product Documents for PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PP2C gamma/PPM1G Antibody (OTI1C12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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