PP2C gamma/PPM1G Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87246

Novus Biologicals, part of Bio-Techne
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NBP1-87246
NBP1-87246-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PP2C gamma Antibodies »

Product Summary for PP2C gamma/PPM1G Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NGELRLLSSIVEELLDQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEGAEENGNSD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2C gamma/PPM1G Antibody

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining in human testis and pancreas tissues using anti-PPM1G antibody. Corresponding PPM1G RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NBP1-87246] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-PPM1G antibody NBP1-87246 (A) shows similar protein distribution across tissues to independent antibody NBP1-87245 (B).
Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87246]

Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87246]

Western Blot: PP2C gamma/PPM1G Antibody [NBP1-87246] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-PPM1G antibody. Remaining relative intensity is presented.
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Applications for PP2C gamma/PPM1G Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C gamma

PPM1G is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Gene Symbol

PPM1G

Additional PP2C gamma Products

Product Documents for PP2C gamma/PPM1G Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PP2C gamma/PPM1G Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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