PP2C gamma/PPM1G Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99758
![Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758]](https://resources.bio-techne.com/images/products/PP2C-gamma-PPM1G-Antibody-Western-Blot-NBP2-99758-img0003.jpg "Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PP2C gamma/PPM1G Antibody
Immunogen
Produced in rabbits immunized with purified, recombinant Human PP2C gamma/PPM1G (Accession#: O15355; Met 317-Asp 546)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PP2C gamma/PPM1G Antibody
Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758]
Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758] - Anti-PP2C gamma/PPM1G rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 292T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 80 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758]](https://resources.bio-techne.com/images/products/PP2C-gamma-PPM1G-Antibody-Immunoprecipitation-NBP2-99758-img0004.jpg "Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758]")
Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758]
Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758] - PP2C gamma/PPM1G was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-PP2C gamma/PPM1G rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PP2C gamma/PPM1G rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDaApplications for PP2C gamma/PPM1G Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
4-6 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PP2C gamma
Long Name
Protein Phosphatase 2C gamma
Alternate Names
PP2CG, PPM1C, PPM1G, PPP2CG
Gene Symbol
PPM1G
Additional PP2C gamma Products
Product Documents for PP2C gamma/PPM1G Antibody
Product Specific Notices for PP2C gamma/PPM1G Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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