PP5 Antibody (12F7)

Western Blot: PP5 Antibody (12F7) [NBP2-59308]

Western Blot: PP5 Antibody (12F7) [NBP2-59308] - Analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Lane 1: MW Ladder. Lane 2: Human A431 (15 ug). Lane 3: Human HEK293 (15 ug). Lane 4: Human Jurkat (15 ug). Load: 15 ug protein. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.

Immunocytochemistry/ Immunofluorescence: PP5 Antibody (12F7) [NBP2-59308]

Immunocytochemistry/Immunofluorescence: PP5 Antibody (12F7) [NBP2-59308] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.

Immunocytochemistry/ Immunofluorescence: PP5 Antibody (12F7) [NBP2-59308]

Immunocytochemistry/Immunofluorescence: PP5 Antibody (12F7) [NBP2-59308] - Tissue: HEK293. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.