PP5 Antibody (OTI2E12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47927
![Flow Cytometry: PP5 Antibody (OTI2E12) [NBP1-47927]](https://resources.bio-techne.com/images/products/PP5-Antibody-2E12-Flow-Cytometry-NBP1-47927-img0001.jpg "Flow Cytometry: PP5 Antibody (OTI2E12) [NBP1-47927]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey, Primate
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2E12
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PPP5C (NP_006238) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens protein phosphatase 5, catalytic subunit (PPP5C).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
56.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PP5 Antibody (OTI2E12)
Western Blot: PP5 Antibody (2E12) [NBP1-47927] Analysis of extracts (35ug) from 9 different cell lines by using anti-PP5 monoclonal antibody.
![Immunocytochemistry/ Immunofluorescence: PP5 Antibody (OTI2E12) [NBP1-47927]](https://resources.bio-techne.com/images/products/PP5-Antibody-2E12-Immunocytochemistry-Immunofluorescence-NBP1-47927-img0003.jpg "Immunocytochemistry/ Immunofluorescence: PP5 Antibody (OTI2E12) [NBP1-47927]")
Immunocytochemistry/ Immunofluorescence: PP5 Antibody (OTI2E12) [NBP1-47927]
Immunocytochemistry/Immunofluorescence: PP5 Antibody (2E12) [NBP1-47927] - Immunofluorescent staining of HT29 cells using anti-PP5 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: PP5 Antibody (OTI2E12) [NBP1-47927]](https://resources.bio-techne.com/images/products/PP5-Antibody-2E12-Immunohistochemistry-Paraffin-NBP1-47927-img0006.jpg "Immunohistochemistry-Paraffin: PP5 Antibody (OTI2E12) [NBP1-47927]")
Immunohistochemistry-Paraffin: PP5 Antibody (OTI2E12) [NBP1-47927]
Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human pancreas tissue using anti-PP5 mouse monoclonal antibody.![Western Blot: PP5 Antibody (OTI2E12) [NBP1-47927]](https://resources.bio-techne.com/images/products/PP5-Antibody-2E12-Western-Blot-NBP1-47927-img0002.jpg "Western Blot: PP5 Antibody (OTI2E12) [NBP1-47927]")
![Immunohistochemistry-Paraffin: PP5 Antibody (OTI2E12) [NBP1-47927]](https://resources.bio-techne.com/images/products/PP5-Antibody-2E12-Immunohistochemistry-Paraffin-NBP1-47927-img0004.jpg "Immunohistochemistry-Paraffin: PP5 Antibody (OTI2E12) [NBP1-47927]")
![Immunohistochemistry-Paraffin: PP5 Antibody (OTI2E12) [NBP1-47927]](https://resources.bio-techne.com/images/products/PP5-Antibody-2E12-Immunohistochemistry-Paraffin-NBP1-47927-img0005.jpg "Immunohistochemistry-Paraffin: PP5 Antibody (OTI2E12) [NBP1-47927]")
Applications for PP5 Antibody (OTI2E12)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PP5
Long Name
Protein Phosphatase 5, Catalytic Subunit
Alternate Names
EC 3.1.3.16, PPP5, PPP5C, PPT
Entrez Gene IDs
5536 (Human)
Gene Symbol
PPP5C
Additional PP5 Products
Product Documents for PP5 Antibody (OTI2E12)
Product Specific Notices for PP5 Antibody (OTI2E12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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