PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52538

Novus Biologicals, part of Bio-Techne
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NBP2-52538-0.025mg
NBP2-52538
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D10C3

Format

BSA Free

Concentration

1 mg/ml

View all PPAR alpha/NR1C1 Antibodies »

Product Summary for PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free

Immunogen

Purified recombinant fragment of human PPAR alpha/NR1C1 (AA: 1-120) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free

Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3)BSA Free [NBP2-52538]

Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3)BSA Free [NBP2-52538]

Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Western blot analysis using PPAR alpha/NR1C1 mouse mAb against HepG2 (1), HEK293 (2), COS7 (7), HepG2 (4), and Jurkat (5) cell lysate.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free [NBP2-52538]

Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free [NBP2-52538]

Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).
Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3)BSA Free [NBP2-52538]

Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3)BSA Free [NBP2-52538]

Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)
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Applications for PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the PPAR alpha is NR1C1.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Gene Symbol

PPARA

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPAR alpha/NR1C1 Antibody (5D10C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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