PPAR alpha/NR1C1 Antibody (OTI1E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01211

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E8

Concentration

1 mg/ml

View all PPAR alpha/NR1C1 Antibodies »

Product Summary for PPAR alpha/NR1C1 Antibody (OTI1E8)

Immunogen

Human recombinant protein fragment corresponding to amino acids 145-420 of human PPARA(NP_005027) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPAR alpha/NR1C1 Antibody (OTI1E8)

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8) [NBP2-01211]

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8) [NBP2-01211]

Western Blot: PPAR alpha/NR1C1 Antibody (1E8) [NBP2-01211] - analysis of PPAR alpha in HeLa cell lysate (25ug) using anti-PPAR alpha antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody (OTI1E8) [NBP2-01211]

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody (OTI1E8) [NBP2-01211]

Immunocytochemistry/Immunofluorescence: PPAR alpha Antibody (1E8) [NBP2-01211] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPAR alpha.
Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8) [NBP2-01211]

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8) [NBP2-01211]

Western Blot: PPAR alpha Antibody (1E8) [NBP2-01211] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAR alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAR alpha.
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Applications for PPAR alpha/NR1C1 Antibody (OTI1E8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq].

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

5465 (Human); 19013 (Mouse)

Gene Symbol

PPARA

UniProt

Q07869

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 Antibody (OTI1E8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPAR alpha/NR1C1 Antibody (OTI1E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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