PPAR alpha/NR1C1 [p Ser12] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-3484

Novus Biologicals, part of Bio-Techne
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NB120-3484
NB120-3484-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all PPAR alpha/NR1C1 Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha.

Modification

p Ser12

Localization

Nuclear

Specificity

NB120-3484 detects phospho-peroxisome proliferator activated receptor (PPAR) alpha S12 from mouse tissues.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAR alpha/NR1C1 [p Ser12] Antibody

Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of PPAR alpha [pSer12] was done on MCF7 cells.
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Applications for PPAR alpha/NR1C1 [p Ser12] Antibody

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Western Blot

1:100 - 1:1000
Application Notes
WB: Detects an approx. 52 kDa protein which corresponds to phospho-PPAR alpha S12 from mouse adipose tissue extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the phospho-PPAR alpha is NR1C1.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

19013 (Mouse)

Gene Symbol

PPARA

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 [p Ser12] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPAR alpha/NR1C1 [p Ser12] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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