PPAR alpha/NR1C1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31834
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Insect, Plant
Predicted:
Canine (92%), Porcine (92%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha/NR1C1. The exact sequence is proprietary.
Reactivity Notes
Queen Bee, Honeybee. Bovine (88%), Guinea pig (87%)
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PPAR alpha/NR1C1 Antibody
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834]](https://resources.bio-techne.com/images/products/PPAR-alpha-NR1C1-Antibody-Western-Blot-NBP1-31834-img0010.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834]")
Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834]
Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Chromatin Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-31834] -
Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-31834] - PPAR alpha/NR1C1 antibody detects PPAR alpha/NR1C1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.PPAR alpha/NR1C1 stained by PPAR alpha/NR1C1 antibody (NBP1-31834) diluted at 1:165.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for PPAR alpha/NR1C1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PPAR alpha/NR1C1
Long Name
Peroxisome Proliferator-activated Receptor alpha
Alternate Names
NR1C1, PPARA
Gene Symbol
PPARA
Additional PPAR alpha/NR1C1 Products
Product Documents for PPAR alpha/NR1C1 Antibody
Product Specific Notices for PPAR alpha/NR1C1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.