PPAR gamma/NR1C3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB120-19481
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Hamster
Cited:
Human, Mouse, Rat, Avian - Chicken, Bovine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to the C terminus of human PPAR gamma.
Localization
Nuclear
Specificity
Will recognize both PPARgamma1 and PPARgamma2
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PPAR gamma/NR1C3 Antibody
Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481]
Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] -
Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. (A) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with pSDS-Dnmt3a1 & pSDS-NC for 48 h are showed; scale bar: 25 μm. (B) Lipid droplet content by oil red O staining & extraction method of cells transfected with pSDS-Dnmt3a1 & pSDS-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗∗P < 0.01. (C) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with si-Dnmt3a1 & si-NC for 48 h are showed; scale bar: 25 μm. (D) Lipid droplet content by oil red O staining & the extraction method of cells transfected with si-Dnmt3a1 & si-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗P < 0.05. (E) Relative mRNA level of some general genes implicated in adipogenesis or energy homeostasis induced by pSDS-Dnmt3a1 & pSDS-NC into cells. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (F) Protein expression of some general adipose genes induced by pSDS-Dnmt3a1 & pSDS-NC into cells. (G) Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 & si-NC into cells for 48 h. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (H) Protein expression of some general genes involved in adipogenesis at 48 h after transfection by si-Dnmt3a1 & si-NC into cells. The band intensity of Western blotting was obtained by averaging the data from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30333755), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PPAR gamma/NR1C3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:400
Application Notes
IHC-P reactivity reported in ( PMID: 25187315).
Reviewed Applications
Read 1 review rated 5 using NB120-19481 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS with 0.2% gelatin
Preservative
0.1% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PPAR gamma/NR1C3
Long Name
Peroxisome Proliferator-activated Receptor gamma
Alternate Names
NR1C3, PPARG
Gene Symbol
PPARG
Additional PPAR gamma/NR1C3 Products
Product Documents for PPAR gamma/NR1C3 Antibody
Product Specific Notices for PPAR gamma/NR1C3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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