PPFIBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25063

Novus Biologicals, part of Bio-Techne
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NBP3-25063
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPFIBP1 Antibodies »

Product Summary for PPFIBP1 Antibody

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein PPFIBP1 using the following amino acid sequence: EEYVCPMELGQASGSASKKGFKPGLDMRLYEEDDLDRLEQMEDSEGTVRQIGAFSEGINNLTHMLKEDDMFKDFAARSPSASITDEDSN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPFIBP1 Antibody

PPFIBP1 Antibody Immunohistochemistry-Paraffin: PPFIBP1 Antibody [NBP3-25063]

Immunohistochemistry-Paraffin: PPFIBP1 Antibody [NBP3-25063]

Staining of human cerebral cortex shows strong positivity in neuropil and cytoplasm of neuronal cells.
PPFIBP1 Antibody Western Blot: PPFIBP1 Antibody [NBP3-25063]

Western Blot: PPFIBP1 Antibody [NBP3-25063]

Analysis in human cell line RT-4 and human cell line U-251 MG.
PPFIBP1 Antibody Immunocytochemistry/Immunofluorescence: PPFIBP1 Antibody [NBP3-25063]

Immunocytochemistry/Immunofluorescence: PPFIBP1 Antibody [NBP3-25063]

Staining of human cell line SK-MEL-30 shows localization to plasma membrane.

Applications for PPFIBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments. For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPFIBP1

PPFIBP1 is encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1).

Alternate Names

hSGT2liprin related protein, hSgt2p, KIAA1230, L2, liprin-beta 1, liprin-beta-1, Protein tyrosine phosphatase receptor type f polypeptide-interactingprotein-binding protein 1, protein-tyrosine phosphatase receptor-type f polypeptide-interactingprotein-binding protein 1, PTPRF interacting protein, binding protein 1 (liprin beta 1), PTPRF-interacting protein-binding protein 1, SGT2

Gene Symbol

PPFIBP1

Additional PPFIBP1 Products

Product Documents for PPFIBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPFIBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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