PPHLN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38431

Novus Biologicals, part of Bio-Techne
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NBP2-38431
NBP2-38431-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPHLN1 Antibodies »

Product Summary for PPHLN1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: STAPLFTDQPEEPESNTTHGIELFEDSQLTTRSKAIASKTKEIEQVYRQDCETFGMVVKMLIEKDPSLEKSIQFALRQNLHEIESAGQTWQQVPPVRNTEMDHDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPHLN1 Antibody

Western Blot: PPHLN1 Antibody [NBP2-38431]

Western Blot: PPHLN1 Antibody [NBP2-38431]

Western Blot: PPHLN1 Antibody [NBP2-38431] - Analysis: NBP2-38431 (A) shows similar pattern to independent antibody NBP1-89480 (B)
Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody [NBP2-38431]

Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody [NBP2-38431]

Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody [NBP2-38431] - Staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP2-38431]

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP2-38431]

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP2-38431] - Staining of human cerebral cortex, colon, liver and testis using Anti-PPHLN1 antibody (A) NBP2-38431 shows similar protein distribution across tissues to independent antibody NBP1-89480 (B).
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Applications for PPHLN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPHLN1

PPHLN1 is encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

Gastric cancer antigen Ga50, HSPC232, MGC48786, periphilin 1, periphilin-1

Gene Symbol

PPHLN1

UniProt

Q8NEY8 (Human)

Additional PPHLN1 Products

Product Documents for PPHLN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPHLN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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