PPIL1 Antibody (08)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06214

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 08

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PPIL1 Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPIL1 (Uniprot#: Q9Y3C6; Met1-Gly166).

Reactivity Notes

No cross-reactivity in ELISA with:

E. coli cell lysate

Specificity

No cross-reactivity in ELISA with: E. coli cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PPIL1 Antibody (08)

Western Blot: PPIL1 Antibody (08) [NBP3-06214]

Western Blot: PPIL1 Antibody (08) [NBP3-06214]

Western Blot: PPIL1 Antibody (08) [NBP3-06214] - Anti-PPIL1 mouse monoclonal antibody at 1:500 dilution. Lane A: U87MG Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:19 kDa(We are unsure as to the identity of these extra bands.)
Flow Cytometry: PPIL1 Antibody (08) [NBP3-06214]

Flow Cytometry: PPIL1 Antibody (08) [NBP3-06214]

Flow Cytometry: PPIL1 Antibody (08) [NBP3-06214] - Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen (TM)), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunoprecipitation: PPIL1 Antibody (08) [NBP3-06214]

Immunoprecipitation: PPIL1 Antibody (08) [NBP3-06214]

Immunoprecipitation: PPIL1 Antibody (08) [NBP3-06214] - PPIL1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg U87MG Whole Cell Lysate 2 uL anti-PPIL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PPIL1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa

Applications for PPIL1 Antibody (08)

Application
Recommended Usage

ELISA

1:1000-1:2000

Flow Cytometry

1:25-1:100

Immunoprecipitation

4-6 ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPIL1

PPIL1 is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Alternate Names

CYPL1cyclophilin-related gene 1, EC 5.2.1.8, hCyPX, MGC678, peptidyl-prolyl cis-trans isomerase, peptidyl-prolyl cis-trans isomerase-like 1, peptidylprolyl isomerase (cyclophilin)-like 1, PPIase, Rotamase PPIL1

Gene Symbol

PPIL1

Additional PPIL1 Products

Product Documents for PPIL1 Antibody (08)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPIL1 Antibody (08)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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