PPM1D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87249

Novus Biologicals, part of Bio-Techne
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NBP1-87249
NBP1-87249-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPM1D Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPM1D Antibody

Immunocytochemistry/ Immunofluorescence: PPM1D Antibody [NBP1-87249]

Immunocytochemistry/ Immunofluorescence: PPM1D Antibody [NBP1-87249]

Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP1-87249] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249]

Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249]

Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249]

Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249]

Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.
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Applications for PPM1D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPM1D

PPM1D is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Long Name

Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D

Alternate Names

PP2C-delta, Wip1

Gene Symbol

PPM1D

Additional PPM1D Products

Product Documents for PPM1D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPM1D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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