PPM1E Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86649

Novus Biologicals, part of Bio-Techne
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NBP1-86649
NBP1-86649-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPM1E Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVKLARSVFSKLHEI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPM1E Antibody

Immunocytochemistry/ Immunofluorescence: PPM1E Antibody [NBP1-86649]

Immunocytochemistry/ Immunofluorescence: PPM1E Antibody [NBP1-86649]

Immunocytochemistry/Immunofluorescence: PPM1E Antibody [NBP1-86649] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: PPM1E Antibody [NBP1-86649]

Immunohistochemistry-Paraffin: PPM1E Antibody [NBP1-86649]

Immunohistochemistry-Paraffin: PPM1E Antibody [NBP1-86649] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.
Immunocytochemistry/ Immunofluorescence: PPM1E Antibody [NBP1-86649]

Immunocytochemistry/ Immunofluorescence: PPM1E Antibody [NBP1-86649]

Immunocytochemistry/Immunofluorescence: PPM1E Antibody [NBP1-86649] - Staining of human cell line U-2 OS shows positivity in nucleus and nucleoli.
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Applications for PPM1E Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPM1E

The protein encoded by the PPM1E gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase was identified as an interacting protein of Rho guanine nucleotide exchange factors (PIX). PIX proteins are regulators of p21/Cdc42/Rac1-activated kinase 1 (PAK1), a protein kinase mediating biological effects downstream of Rho GTPases. This phosphatase has been shown to block the effects of PAK, and thus inhibit actin stress fiber breakdown and morphological changes driven by cell division cycle 42 (CDC42). (provided by RefSeq)

Alternate Names

ca(2+)/calmodulin-dependent protein kinase phosphatase N, CAMKN, CaMKP-N, caMKP-nucleus, DKFZp781F1422, KIAA1072, nuclear calmodulin-dependent protein kinase phosphatase, partner of PIX 1, partner of PIXA, partner of PIX-alpha, POPX1, PP2CH, protein phosphatase 1E, protein phosphatase 1E (PP2C domain containing), protein phosphatase, Mg2+/Mn2+ dependent, 1E

Gene Symbol

PPM1E

Additional PPM1E Products

Product Documents for PPM1E Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPM1E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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