PPME1 Antibody (OTI7F12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02240

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7F12

Concentration

0.96 mg/ml

View all PPME1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPME1 Antibody (OTI7F12)

Western Blot: PPME1 Antibody (OTI7F12) [NBP2-02240]

Western Blot: PPME1 Antibody (OTI7F12) [NBP2-02240]

Western Blot: PPME1 Antibody (7F12) [NBP2-02240] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
Immunocytochemistry/Immunofluorescence: PPME1 Antibody (7F12) [NBP2-02240] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1.
Immunohistochemistry-Paraffin: PPME1 Antibody (OTI7F12) [NBP2-02240]

Immunohistochemistry-Paraffin: PPME1 Antibody (OTI7F12) [NBP2-02240]

Immunohistochemistry-Paraffin: PPME1 Antibody (7F12) [NBP2-02240] - Staining of paraffin-embedded Kidney tissue using anti-PPME1mouse monoclonal antibody.
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Applications for PPME1 Antibody (OTI7F12)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.96 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPME1

Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit

Alternate Names

EC 3.1.1, EC 3.1.1.-, FLJ22226, PME-1PME1, protein phosphatase methylesterase 1, protein phosphatase methylesterase-1

Entrez Gene IDs

51400 (Human)

Gene Symbol

PPME1

Additional PPME1 Products

Product Documents for PPME1 Antibody (OTI7F12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPME1 Antibody (OTI7F12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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