PPME1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38515

Novus Biologicals, part of Bio-Techne
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NBP2-38515
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPME1 Antibodies »

Product Summary for PPME1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPME1 Antibody

Western Blot: PPME1 Antibody [NBP2-38515]

Western Blot: PPME1 Antibody [NBP2-38515]

Western Blot: PPME1 Antibody [NBP2-38515] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line CACO-2
Immunohistochemistry-Paraffin: PPME1 Antibody [NBP2-38515]

Immunohistochemistry-Paraffin: PPME1 Antibody [NBP2-38515]

Immunohistochemistry-Paraffin: PPME1 Antibody [NBP2-38515] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Simple Western: PPME1 Antibody [NBP2-38515]

Simple Western: PPME1 Antibody [NBP2-38515]

Simple Western: PPME1 Antibody [NBP2-38515] - Simple Western lane view shows a specific band for PPME1 in 0.2 mg/ml of HT-29 (left) and HCT 116 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
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Applications for PPME1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Simple Western: PPME1 antibody was used at 1:25 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPME1

Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit (PPP2CA; MIM 176915) (Ogris et al., 1999 [PubMed 10318862]).[supplied by OMIM]

Alternate Names

EC 3.1.1, EC 3.1.1.-, FLJ22226, PME-1PME1, protein phosphatase methylesterase 1, protein phosphatase methylesterase-1

Gene Symbol

PPME1

UniProt

Q9Y570 (Human)

Additional PPME1 Products

Product Documents for PPME1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPME1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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