PPP2R1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19907

Novus Biologicals, part of Bio-Techne
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NBP2-19907
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPP2R1A Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (88%), Xenopus laevis (95%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPP2R1A Antibody

Western Blot: PPP2R1A Antibody [NBP2-19907]

Western Blot: PPP2R1A Antibody [NBP2-19907]

Western Blot: PPP2R1A Antibody [NBP2-19907] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP2R1A antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PPP2R1A Antibody [NBP2-19907]

Immunocytochemistry/ Immunofluorescence: PPP2R1A Antibody [NBP2-19907]

Immunocytochemistry/Immunofluorescence: PPP2R1A Antibody [NBP2-19907] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: PPP2R1A Antibody [NBP2-19907]

Western Blot: PPP2R1A Antibody [NBP2-19907]

Western Blot: PPP2R1A Antibody [NBP2-19907] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:5000.
View 1 more images

Applications for PPP2R1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:2500

Immunohistochemistry-Paraffin

1:100-1:2500

Western Blot

1:500-1:10000
Application Notes
Immunoprecipitation Assay-dependent dilution

Reviewed Applications

Read 1 review rated 4 using NBP2-19907 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPP2R1A

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq]

Alternate Names

Medium tumor antigen-associated 61 kDa protein, MGC786, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PP2AAALPHA, PP2A-Aalpha, PR65A, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, protein phosphatase 2, regulatory subunit A, alpha, serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform

Gene Symbol

PPP2R1A

UniProt

P30153 (Human)

Additional PPP2R1A Products

Product Documents for PPP2R1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPP2R1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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