PPP2R3A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93429

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PPP2R3A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QKDVENDGPEPSD corresponding to internal region according to NP_002709.2, NP_871626.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PPP2R3A Antibody

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NB100-93429]

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NB100-93429]

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NB100-93429] - Staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PPP2R3A Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 80 kDa band in human skeletal muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130 kDa band according to NP_002709.2 and of 61.1 kDa band according to NP_871626.1. The 80 kDa band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP2R3A

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

PP2A subunit B isoform R3 isoform, PP2A subunit B isoforms B72/B130, PP2A subunit B isoforms B'-PR72/PR130, protein phosphatase 2 (formerly 2A), regulatory subunit B' (PR 72), alphaisoform and (PR 130), beta isoform, protein phosphatase 2 (formerly 2A), regulatory subunit B', alpha, protein phosphatase 2, regulatory subunit B', alpha, serine/threonine-protein phosphatase 2A regulatory subunit B' subunit alpha, subunit B, R3 isoform

Entrez Gene IDs

5523 (Human)

Gene Symbol

PPP2R3A

UniProt

Q06190 (Human)

Additional PPP2R3A Products

Product Documents for PPP2R3A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPP2R3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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