PPP3CB Antibody (5D3)
Novus Biologicals, part of Bio-Techne | Catalog # H00005532-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5D3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PPP3CB (NP_066955, 435 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LTPTGMLPSGVLAGGRQTLQSATVEAIEAEKAIRGFSPPHRICSFEEAKGLDRINERMPPRKDAVQQDGFNSLNTAHATENHGTGNHTAQ
Specificity
PPP3CB - protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PPP3CB Antibody (5D3)
![Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]](https://resources.bio-techne.com/images/products/PPP3CB-Antibody-5D3-Western-Blot-H00005532-M01-img0006.jpg "Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]")
Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]
Western Blot: PPP3CB Antibody (5D3) [H00005532-M01] - Analysis of PPP3CB expression in transfected 293T cell line by PPP3CB monoclonal antibody (M01), clone 5D3.Lane 1: PPP3CB transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.![Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]](https://resources.bio-techne.com/images/products/PPP3CB-Antibody-5D3-Western-Blot-H00005532-M01-img0004.jpg "Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]")
Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]
Western Blot: PPP3CB Antibody (5D3) [H00005532-M01] - Analysis of PPP3CB over-expressed 293 cell line, cotransfected with PPP3CB Validated Chimera RNAi ( Cat # H00005532-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPP3CB monoclonal antibody (M01), clone 5D3 (Cat # H00005532-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: PPP3CB Antibody (5D3) [H00005532-M01] - Detection limit for recombinant GST tagged PPP3CB is approximately 0.03ng/ml as a capture antibody.
Applications for PPP3CB Antibody (5D3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PPP3CB
Long Name
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
Alternate Names
CALNA2, CALNB, CNA beta, CNA2
Entrez Gene IDs
5532 (Human)
Gene Symbol
PPP3CB
OMIM
114106 (Human)
UniProt
Additional PPP3CB Products
Product Documents for PPP3CB Antibody (5D3)
Product Specific Notices for PPP3CB Antibody (5D3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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