PRA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80883

Novus Biologicals, part of Bio-Techne
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NBP1-80883
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRA1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAAQKDQQKDAEAEGLSGTTLLPKLIPSGAGREWLERRRATIRPWSTFVDQQRFSRP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRA1 Antibody

Western Blot: PRA1 Antibody [NBP1-80883]

Western Blot: PRA1 Antibody [NBP1-80883]

Western Blot: PRA1 Antibody [NBP1-80883] - Analysis in control (vector only transfected HEK293T lysate) and RABAC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PRA1 Antibody [NBP1-80883]

Immunocytochemistry/ Immunofluorescence: PRA1 Antibody [NBP1-80883]

Immunocytochemistry/Immunofluorescence: PRA1 Antibody [NBP1-80883] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center and nuclear membrane.
Immunohistochemistry-Paraffin: PRA1 Antibody [NBP1-80883]

Immunohistochemistry-Paraffin: PRA1 Antibody [NBP1-80883]

Immunohistochemistry-Paraffin: PRA1 Antibody [NBP1-80883] - Staining in human cerebral cortex and liver tissues using anti-RABAC1 antibody. Corresponding RABAC1 RNA-seq data are presented for the same tissues.
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Applications for PRA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRA1

PRA1 (prenylated Rab acceptor) is a general regulator of Rab proteins. It has been shown that PRA1 interacts with Rab proteins and with VAMP2. Therefore PRA1 is probably an important factor for membrane traffic, linking together the function of Rab proteins and SNAREs (1). Human cells contain more than 60 small G proteins of the Rab family, which are localized to the surfaces of distinct membrane compartments and regulate transport vesicle formation, motility, docking and fusion. Prenylated Rabs also occur in the cytosol bound to GDI (guanine nucleotide dissociation inhibitor), which binds to Rabs in their inactive state (2).

Alternate Names

PRA1 family protein 1, PRA1PRA1 domain family 1, PRAF1prenylated Rab acceptor 1, prenylated Rab acceptor protein 1, Rab acceptor 1 (prenylated), YIP3

Entrez Gene IDs

10567 (Human)

Gene Symbol

RABAC1

UniProt

Q9UI14 (Human)

Additional PRA1 Products

Product Documents for PRA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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