PRAM1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-821
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Porcine (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence DFCDPLENQPLPLGR corresponding to C-Terminus according to NP_115528.4.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PRAM1 Antibody
![Western Blot: PRAM1 Antibody [NB100-821]](https://resources.bio-techne.com/images/products/PRAM1-Antibody-Western-Blot-NB100-821-img0003.jpg "Western Blot: PRAM1 Antibody [NB100-821]")
Western Blot: PRAM1 Antibody [NB100-821]
Western Blot: PRAM1 Antibody [NB100-821] - Analysis of PRAM1 in Jurkat lysate using this antibody at 2 ug/ ml. Primary incubated for 12 hour. Detected using chemiluminescence.Applications for PRAM1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
1:100 - 1:2000
Application Notes
WB: Approx. 90-100 kDa band observed in Jurkat lysates. Overnight incubation is recommended with this antibody. An 79 kDa band of PRAM-1 (predicted MW of 79 kDa band) and detected a migration with an apparent MW of 97 kDa is in agreement with scientific literature (Moog-Lutz et al., J. Biol. Chem, Jun 2001; 276: 22375 - 22381).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PRAM1
Alternate Names
MGC39864, PML-RAR, PML-RARA regulated adaptor molecule 1, PML-RARA target gene encoding an Adaptor Molecule-1, PML-RARA-regulated adapter molecule 1, PRAM, PRAM-1
Entrez Gene IDs
84106 (Human)
Gene Symbol
PRAM1
UniProt
Additional PRAM1 Products
Product Documents for PRAM1 Antibody
Product Specific Notices for PRAM1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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