Pref-1/DLK1/FA1 Antibody (PF299-1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80047
![Western Blot: Pref-1/DLK1/FA1 Antibody (PF299-1)Azide and BSA Free [NBP2-80047]](https://resources.bio-techne.com/images/products/Pref-1-DLK1-FA1-Antibody-PF299-1-Western-Blot-NBP2-80047-img0003.jpg "Western Blot: Pref-1/DLK1/FA1 Antibody (PF299-1)Azide and BSA Free [NBP2-80047]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PF299-1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Pref-1/DLK1/FA1 Antibody (PF299-1) - Azide and BSA Free
Immunogen
Recombinant human Pref-1/DLK1/FA1 (extracellular domain).
Specificity
Recognizes human Pref-1/DLK1/FA1. Does not cross-react with mouse Pref-1/DLK1/FA1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Pref-1/DLK1/FA1 Antibody (PF299-1) - Azide and BSA Free
Western Blot: Pref-1/DLK1/FA1 Antibody (PF299-1)Azide and BSA Free [NBP2-80047]
Western Blot: Pref-1/DLK1/FA1 Antibody (PF299-1) [NBP2-80047] - Analysis of human DLK1 using anti-DLK1 (human), mAb (PF299-1) at 1: 2,000 dilution.Panel A. 1. Human DLK1-Fc protein. 2. Transfected human DLK1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate.Panel B. 1. Human DLK1-Fc protein. 2. Mouse DLK1-Fc protein. 3. Human DNER-Fc protein. 4. Human DLL1-Fc protein. 5. Human DLL3-Fc protein. 6. Human-DLL4 Fc protein. 7. Human Jagged1-Fc protein. 8. Human FGF23-Fc protein.
Pref-1/DLK1/FA1 Antibody (PF299-1) [NBP2-80047] - Immunohistochemical staining of DLK1 using anti-DLK1 (human), mAb (PF299-1) in placenta, villi (1:500 dilution). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Pref-1/DLK1/FA1 Antibody (PF299-1) [NBP2-80047] - FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF299-1).
Applications for Pref-1/DLK1/FA1 Antibody (PF299-1) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:2000 - 1:10000
Flow Cytometry
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Western Blot
1:2000 - 1:10000
Application Notes
Western blot: 1:2000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um-filtered solution in PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pref-1/DLK1/FA1
Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Alternate Names
DLK-1, DLK1, FA1, pG2, Pref1, ZOG
Gene Symbol
DLK1
Additional Pref-1/DLK1/FA1 Products
Product Documents for Pref-1/DLK1/FA1 Antibody (PF299-1) - Azide and BSA Free
Product Specific Notices for Pref-1/DLK1/FA1 Antibody (PF299-1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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