PRKAG2 Antibody (3C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00051422-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRKAG2 Antibodies »

Product Specifications

Immunogen

PRKAG2 (AAH20540, 191 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAE

Reactivity Notes

Human. Other species not tested.

Specificity

PRKAG2 - protein kinase, AMP-activated, gamma 2 non-catalytic subunit

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PRKAG2 Antibody (3C4)

Western Blot: PRKAG2 Antibody (3C4) [H00051422-M01]

Western Blot: PRKAG2 Antibody (3C4) [H00051422-M01]

Western Blot: PRKAG2 Antibody (3C4) [H00051422-M01] - Analysis of PRKAG2 expression in transfected 293T cell line by PRKAG2 monoclonal antibody (M01), clone 3C4.Lane 1: PRKAG2 transfected lysate (Predicted MW: 37.5 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PRKAG2 Antibody (3C4) [H00051422-M01]

Immunocytochemistry/ Immunofluorescence: PRKAG2 Antibody (3C4) [H00051422-M01]

Immunocytochemistry/Immunofluorescence: PRKAG2 Antibody (3C4) [H00051422-M01] - Analysis of monoclonal antibody to PRKAG2 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: PRKAG2 Antibody (3C4) [H00051422-M01] - Detection limit for recombinant GST tagged PRKAG2 is approximately 0.03ng/ml as a capture antibody.

Applications for PRKAG2 Antibody (3C4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein on ELISA. GST alone used as a negative control. It has also been used for immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRKAG2

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

5'-AMP-activated protein kinase subunit gamma-2

Alternate Names

AAKG, AAKG2, AMPK gamma2, CMH6, H91620p, WPWS

Entrez Gene IDs

51422 (Human)

Gene Symbol

PRKAG2

OMIM

194 (Human)

UniProt

AAH20540 (Human)

Additional PRKAG2 Products

Product Documents for PRKAG2 Antibody (3C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRKAG2 Antibody (3C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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