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PRKCDBP Antibody (8D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00112464-M04

Novus Biologicals, part of Bio-Techne
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H00112464-M04

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PRKCDBP (NP_659477, 161 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVGESSDEEPVESRAQRLRRTGLQKVQSLRRALSGRKGPAAPPPTPVKPPRLGPGRSAEAQPEAQPALEPTLEPEPPQDTEEDPGRPGAAEEALLQMESVA

Specificity

This product is specific for Human PRKCDBP monoclonal antibody (M04), clone 8D3 [Gene ID: 112464].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PRKCDBP Antibody (8D3)

Western Blot: PRKCDBP Antibody (8D3) [H00112464-M04]

Western Blot: PRKCDBP Antibody (8D3) [H00112464-M04]

Western Blot: PRKCDBP Antibody (8D3) [H00112464-M04] - Analysis of PRKCDBP expression in transfected 293T cell line by PRKCDBP monoclonal antibody (M04), clone 8D3. Lane 1: PRKCDBP transfected lysate (Predicted MW: 27.6 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: PRKCDBP Antibody (8D3) [H00112464-M04] - Detection limit for recombinant GST tagged PRKCDBP is 0.3 ng/ml as a capture antibody.

Applications for PRKCDBP Antibody (8D3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Mouse monoclonal antibody raised against a partial recombinant PRKCDBP. Use in ICC/IF was reported in scientific literature (PMID: 31332168).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRKCDBP

PRKCDBP, also known as Protein kinase C delta-binding protein, is 261 amino acids in length and approximately 27kDa. PRKCDBP is highly expressed in epithelial cells, and is down regulated in lung cancer cell lines and breast cancer cell lines, thus suggesting the possibility of tumor suppressor function. PRKCDBP binds and is phosphorylated by protein kinase C, delta. Current research surrounding PRKCDBP shows a possible connection with ovarian cancer, gastric cancer and lung cancer. PRKCDBP has also been shown to interact with Caveolin 1, Caveolin 3, cMyc, SDPR, and EPS15L1.

Alternate Names

cavin-3, HSRBC, MGC20400, protein kinase C, delta binding protein, sdr-related gene product that binds to c-kinase, Serum deprivation response factor-related gene product that binds to C-kinase, SRBCprotein kinase C delta-binding protein

Gene Symbol

CAVIN3

Additional PRKCDBP Products

Product Documents for PRKCDBP Antibody (8D3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRKCDBP Antibody (8D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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