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PRKCDBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44091

Novus Biologicals, part of Bio-Techne
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NBP1-44091

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PRKCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human PRKCDBP. (Uniprot: Q969G5)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with PRKCDBP from other sources has not been determined.

Specificity

A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with PRKCDBP from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for PRKCDBP Antibody

Immunohistochemistry: PRKCDBP Antibody [NBP1-44091]

Immunohistochemistry: PRKCDBP Antibody [NBP1-44091]

Immunohistochemistry: PRKCDBP Antibody [NBP1-44091] - Tissue: Placenta. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Protein Kinase C delta Binding Protein at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Protein Kinase C delta Binding Protein as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry: PRKCDBP Antibody [NBP1-44091]

Immunohistochemistry: PRKCDBP Antibody [NBP1-44091]

Immunohistochemistry: PRKCDBP Antibody [NBP1-44091] - Tissue: Lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.

Applications for PRKCDBP Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

5 ug/ml

Western Blot

1ug/ml
Application Notes
This product is tested in immunohistochemistry and ELISA and useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~37kDa corresponding to the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: PRKCDBP

The protein encoded by this gene was identified as a binding protein of the protein kinase C, delta (PRKCD). The expression of this gene in cultured cell lines is strongly induced by serum starvation. The expression of this protein was found to be down-regulated in various cancer cell lines, suggesting the possible tumor suppressor function of this protein.

Alternate Names

cavin-3, HSRBC, MGC20400, protein kinase C, delta binding protein, sdr-related gene product that binds to c-kinase, Serum deprivation response factor-related gene product that binds to C-kinase, SRBCprotein kinase C delta-binding protein

Entrez Gene IDs

112464 (Human)

Gene Symbol

CAVIN3

UniProt

Additional PRKCDBP Products

Product Documents for PRKCDBP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRKCDBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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