PRKCDBP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45210
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
PRKCDBP Antibody is made to a peptide with sequence C-RRTGLQKVQSLR corresponding to internal region according to NP_659477.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PRKCDBP Antibody
Western Blot: PRKCDBP Antibody [NBP1-45210]
Western Blot: PRKCDBP Antibody [NBP1-45210] - Staining of Mouse Ovary (A) and Rat Uterus (B) lysate (35 ug protein in RIPA buffer). PRKCDBP antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP1-45210]
Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP1-45210] - Staining of paraffin embedded Human Uterus. PRKCDBP antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Western Blot: PRKCDBP Antibody [NBP1-45210]
Western Blot: PRKCDBP Antibody [NBP1-45210] - Staining of Human Adipose cell llysate (35 ug protein in RIPA buffer). PRKCDBP antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for PRKCDBP Antibody
Application
Recommended Usage
Immunohistochemistry
3.75 ug/mL
Immunohistochemistry-Paraffin
3.75 ug/mL
Peptide ELISA
Detection limit 1:32000
Western Blot
0.1 - 1 ug/mL
Application Notes
Approx 38 kDa band observed in Human Adipose, in Mouse Ovary and Rat Uterus and in NIH3T3 lysates (calculated MW of 27.7 kDa according to Human NP_659477.2, of 27.8 kDa according to Mouse NP_082720.1 and of 27.9 kDa according to Rat NP_604444.1).The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PRKCDBP
Alternate Names
cavin-3, HSRBC, MGC20400, protein kinase C, delta binding protein, sdr-related gene product that binds to c-kinase, Serum deprivation response factor-related gene product that binds to C-kinase, SRBCprotein kinase C delta-binding protein
Gene Symbol
CAVIN3
UniProt
Additional PRKCDBP Products
Product Documents for PRKCDBP Antibody
Product Specific Notices for PRKCDBP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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