PRKCSH Antibody (A6H9-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32386
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A6H9-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human PRKCSH aa 51-250. (Uniprot: P14314)
Localization
Endoplasmic reticulum.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PRKCSH Antibody (A6H9-R)
Western Blot: PRKCSH Antibody (A6H9-R) [NBP3-32386]
Western Blot: PRKCSH Antibody (A6H9-R) [NBP3-32386] - Western blot analysis of PRKCSH on different lysates with Mouse anti-PRKCSH antibody (NBP3-32386) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Daudi cell lysate
Lane 4: Jurkat cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 59 kDa
Observed band size: 80 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32386) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for PRKCSH Antibody (A6H9-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PRKCSH
Alternate Names
AGE-R2,80K-H protein, G19P1AGE-binding receptor 2, glucosidase 2 subunit beta, Glucosidase II subunit beta, glucosidase II, beta subunit, hepatocystin, PCLD, PKCSH, PLD1, Protein kinase C substrate 60.1 kDa protein heavy chain, protein kinase C substrate 80K-H, protein kinase C substrate, 80 Kda protein
Gene Symbol
PRKCSH
Additional PRKCSH Products
Product Documents for PRKCSH Antibody (A6H9-R)
Product Specific Notices for PRKCSH Antibody (A6H9-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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