PRMT5 Antibody (3610)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43678

Novus Biologicals, part of Bio-Techne
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NBP2-43678
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (98%), Mouse (98%), Porcine (100%), Primate (99%), Rhesus Macaque (100%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3610

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRMT5 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT5. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%), Japanese medaka (86%), Xenopus laevis (91%), Chimpanzee (99%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRMT5 Antibody (3610)

Western Blot: PRMT5 Antibody (3610) [NBP2-43678]

Western Blot: PRMT5 Antibody (3610) [NBP2-43678]

Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE PRMT5 antibody [3610] dilution: 1:1000.
Immunoprecipitation: PRMT5 Antibody (3610) [NBP2-43678]

Immunoprecipitation: PRMT5 Antibody (3610) [NBP2-43678]

Immunoprecipitation: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of Raji whole cell extract A. 35 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [3610] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [3610] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
PRMT5 Antibody (3610)

Western Blot: PRMT5 Antibody (3610) [NBP2-43678] -

Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (NBP2-43678) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for PRMT5 Antibody (3610)

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRMT5

Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Long Name

Protein arginine N-methyltransferase 5

Alternate Names

EC 2.1.1.320, HRMT1L5, IBP72, JBP1, SKB1, SKB1 homolog

Gene Symbol

PRMT5

Additional PRMT5 Products

Product Documents for PRMT5 Antibody (3610)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRMT5 Antibody (3610)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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