Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PRMT6 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 325 and the C-terminus (residue 375) of human Protein Arginine Methyltransferase 6 using the numbering given in entry AAK85733.1 (GeneID 55170).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRMT6 Antibody - BSA Free

Western Blot: PRMT6 Antibody [NB110-40713]

Western Blot: PRMT6 Antibody [NB110-40713]

Western Blot: PRMT6 Antibody [NB110-40713] - Detection of Human PRMT6 on HeLa whole cell lysate using NB110-40713. PRMT6 was also immunoprecipitated by rabbit anti-PRMT6 antibody NB110-40712.
Immunohistochemistry-Paraffin: PRMT6 Antibody [NB110-40713]

Immunohistochemistry-Paraffin: PRMT6 Antibody [NB110-40713]

Immunohistochemistry-Paraffin: PRMT6 Antibody [NB110-40713] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- PRMT6 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB

Applications for PRMT6 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Western Blot

1:2000-1:10000
Application Notes
Immunoprecipitation is not recommended, use NB 110-40712. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRMT6

PRMT6 severely impairs the function of HIV-1 Rev. PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. In addition, PRMT6 displays automethylation activity; it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication.

Long Name

Protein arginine N-methyltransferase 6

Alternate Names

HRMT1L6

Entrez Gene IDs

55170 (Human)

Gene Symbol

PRMT6

UniProt

AAK85733.1 (Human)

Additional PRMT6 Products

Product Documents for PRMT6 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRMT6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.