PRMT6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99773

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PRMT6 Antibodies »

Product Summary for PRMT6 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human PRMT6 (Accession#: NP_060607.2; Met 1-Asp 375)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PRMT6 Antibody

Western Blot: PRMT6 Antibody [NBP2-99773]

Western Blot: PRMT6 Antibody [NBP2-99773]

Western Blot: PRMT6 Antibody [NBP2-99773] - Anti-PRMT6 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa
Western Blot: PRMT6 Antibody [NBP2-99773]

Western Blot: PRMT6 Antibody [NBP2-99773]

Western Blot: PRMT6 Antibody [NBP2-99773] - Anti-PRMT6 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa
Immunoprecipitation: PRMT6 Antibody [NBP2-99773]

Immunoprecipitation: PRMT6 Antibody [NBP2-99773]

Immunoprecipitation: PRMT6 Antibody [NBP2-99773] - PRMT6 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. Lane C: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-PRMT6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PRMT6 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa

Applications for PRMT6 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRMT6

PRMT6 severely impairs the function of HIV-1 Rev. PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. In addition, PRMT6 displays automethylation activity; it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication.

Long Name

Protein arginine N-methyltransferase 6

Alternate Names

HRMT1L6

Gene Symbol

PRMT6

Additional PRMT6 Products

Product Documents for PRMT6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRMT6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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