Profilin 1 Antibody (CL3524) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43957

Novus Biologicals, part of Bio-Techne
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NBP3-43957
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BSA Free
NBP2-59778
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL3524

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Profilin 1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07737, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Profilin 1 Antibody (CL3524) - Azide and BSA Free

Profilin 1 Antibody (CL3524) - Azide and BSA Free Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP3-43957]

Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP3-43957]

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Profilin 1 Antibody (CL3524) - Azide and BSA Free Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP3-43957]

Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP3-43957]

Staining of human liver shows strong cytoplasmic positivity in leukocytes.
Profilin 1 Antibody (CL3524) - Azide and BSA Free Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP3-43957]

Immunohistochemistry-Paraffin: Profilin 1 Antibody [NBP3-43957]

Staining of human testis shows very weak cytoplasmic positivity in cells in seminiferous ducts.
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Applications for Profilin 1 Antibody (CL3524) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 Antibody (CL3524) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Profilin 1 Antibody (CL3524) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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