Profilin 1 Antibody (OTI1D5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73605

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Profilin 1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

14.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Profilin 1 Antibody (OTI1D5) - Azide and BSA Free

Western Blot: Profilin 1 Antibody (OTI1D5)Azide and BSA Free [NBP2-73605]

Western Blot: Profilin 1 Antibody (OTI1D5)Azide and BSA Free [NBP2-73605]

Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.
Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605]

Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605]

Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.
Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605]

Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605]

Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.
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Applications for Profilin 1 Antibody (OTI1D5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 Antibody (OTI1D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Profilin 1 Antibody (OTI1D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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