Profilin 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99325
![Western Blot: Profilin 1 Antibody [NBP2-99325]](https://resources.bio-techne.com/images/products/Profilin-1-Antibody-Western-Blot-NBP2-99325-img0002.jpg "Western Blot: Profilin 1 Antibody [NBP2-99325]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Profilin 1 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Profilin 1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Profilin 1 Antibody
Western Blot: Profilin 1 Antibody [NBP2-99325]
Western Blot: Profilin 1 Antibody [NBP2-99325] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa![Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody [NBP2-99325]](https://resources.bio-techne.com/images/products/Profilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99325-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody [NBP2-99325]")
Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody [NBP2-99325]
Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP2-99325] - Immunofluorescence staining of Profilin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Profilin 1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm.Applications for Profilin 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Profilin 1
Alternate Names
PFN1, PROF1, Profilin I
Gene Symbol
PFN1
Additional Profilin 1 Products
Product Documents for Profilin 1 Antibody
Product Specific Notices for Profilin 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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