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Profilin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31629

Novus Biologicals, part of Bio-Techne
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NBP1-31629

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%), Chicken (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Profilin 2. The exact sequence is proprietary.

Localization

Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Profilin 2 Antibody

Western Blot: Profilin 2 Antibody [NBP1-31629]

Western Blot: Profilin 2 Antibody [NBP1-31629]

Western Blot: Profilin 2 Antibody [NBP1-31629] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 2 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-31629]

Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-31629]

Immunohistochemistry-Paraffin: Profilin 2 Antibody [NBP1-31629] - Human colon carcinoma. Profilin 2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Profilin 2 Antibody [NBP1-31629]

Western Blot: Profilin 2 Antibody [NBP1-31629]

Western Blot: Profilin 2 Antibody [NBP1-31629] - Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE; antibody diluted at 1:1000.

Applications for Profilin 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Profilin 2

Profilin 2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals by binding to actin (1:1 ratio) and so affecting the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Highly expressed in skeletal muscle, kidney and brain and less strongly in placenta, heart, liver and lung.

Alternate Names

D3S1319E, PFL, profilin 2, Profilin II, profilin-2

Gene Symbol

PFN2

Additional Profilin 2 Products

Product Documents for Profilin 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Profilin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.