Progesterone R/NR3C3 [p Ser294] Antibody (608)
Novus Biologicals, part of Bio-Techne | Catalog # NB200-320
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 608
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH. Accession # P06401
Modification
p Ser294
Localization
Nuclear
Specificity
Specific for endogenous levels of the ~90 kDa Progesterone R/NR3C3-A isoform and the ~120 kDa Progesterone R/NR3C3-B isoform phosphorylated at Ser294. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Progesterone R/NR3C3 [p Ser294] Antibody (608)
Western Blot: Progesterone Receptor [p Ser294] Antibody (608) [NB200-320] - Whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).
Applications for Progesterone R/NR3C3 [p Ser294] Antibody (608)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:1000
Western Blot
1:1000
Application Notes
Recognizes a ~115 kDa doublet/triplet and also a 80 kDa single band in western blots.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Progesterone R/NR3C3
Long Name
Progesterone Receptor
Alternate Names
NR3C3, PGR, ProgesteroneR
Entrez Gene IDs
5241 (Human)
Gene Symbol
PGR
UniProt
Additional Progesterone R/NR3C3 Products
Product Documents for Progesterone R/NR3C3 [p Ser294] Antibody (608)
Product Specific Notices for Progesterone R/NR3C3 [p Ser294] Antibody (608)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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