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Progesterone R/NR3C3 Antibody (PGR/8099R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23941

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-23941-100ug
NBP3-23941-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PGR/8099R

Concentration

0.2 mg/ml

Product Summary for Progesterone R/NR3C3 Antibody (PGR/8099R)

Immunogen

Recombinant fragment (around aa 1-200) of human Progesterone R/NR3C3 (exact sequence is proprietary)

Localization

Nucleus.

Marker

Marker of Progestin Dependence

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for Progesterone R/NR3C3 Antibody (PGR/8099R)

Progesterone R/NR3C3 Antibody (PGR/8099R)

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PGR/8099R) [NBP3-23941] -

Formalin-fixed, paraffin-embedded human breast cancer stained with Progesterone R/NR3C3 antibody (PGR/8099R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Progesterone R/NR3C3 Antibody (PGR/8099R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Progesterone R/NR3C3

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Long Name

Progesterone Receptor

Alternate Names

NR3C3, PGR, ProgesteroneR

Gene Symbol

PGR

Additional Progesterone R/NR3C3 Products

Product Documents for Progesterone R/NR3C3 Antibody (PGR/8099R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Progesterone R/NR3C3 Antibody (PGR/8099R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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