Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33369
![Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free [NBP2-33369]](https://resources.bio-techne.com/images/products/Progesterone-R-NR3C3-Antibody-PR501-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33369-img0001.jpg "Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free [NBP2-33369]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PR501
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human Progesterone R/NR3C3 protein (Uniprot: P06401)
Localization
Nucleus
Specificity
This monoclonal antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone.
Marker
Marker of Progestin Dependence
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-33368).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free
Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free [NBP2-33369]
Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free [NBP2-33369] - Formalin-paraffin normal human breast (left) and invasive ductal carcinoma (right) stained with progesterone receptor Ab (PR501).Applications for Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free
Application
Recommended Usage
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Progesterone R/NR3C3
Long Name
Progesterone Receptor
Alternate Names
NR3C3, PGR, ProgesteroneR
Gene Symbol
PGR
Additional Progesterone R/NR3C3 Products
Product Documents for Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free
Product Specific Notices for Progesterone R/NR3C3 Antibody (PR501) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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