Progesterone R/NR3C3 Antibody (SPM566)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34382
![Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SPM566) [NBP2-34382]](https://resources.bio-techne.com/images/products/Progesterone-R-NR3C3-Antibody-SPM566-Immunohistochemistry-Paraffin-NBP2-34382-img0001.jpg "Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SPM566) [NBP2-34382]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM566
Concentration
0.2 mg/ml
Product Summary for Progesterone R/NR3C3 Antibody (SPM566)
Immunogen
Recombinant full-length human Progesterone R/NR3C3 protein (PGR) (Uniprot: P06401 )
Localization
Nuclear
Specificity
This monoclonal antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues.
Marker
Marker of Progestin Dependence
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34429)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Progesterone R/NR3C3 Antibody (SPM566)
Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SPM566) [NBP2-34382]
Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (SPM566) [NBP2-34382] - Formalin-paraffin normal human breast carcinoma stained with Progesterone Receptor MAb (SPM566).Applications for Progesterone R/NR3C3 Antibody (SPM566)
Application
Recommended Usage
Immunohistochemistry
-frozen 0.5-1.0ug/ml
Immunohistochemistry-Frozen
0.5-1.0ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Progesterone R/NR3C3
Long Name
Progesterone Receptor
Alternate Names
NR3C3, PGR, ProgesteroneR
Gene Symbol
PGR
UniProt
Additional Progesterone R/NR3C3 Products
Product Documents for Progesterone R/NR3C3 Antibody (SPM566)
Product Specific Notices for Progesterone R/NR3C3 Antibody (SPM566)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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