Prohibitin Antibody (0V2R10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15756

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15756-100ul
NBP3-15756-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0V2R10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Prohibitin Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Prohibitin (P35232). ASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Prohibitin Antibody (0V2R10)

Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756]

Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756]

Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756] - Western blot analysis of extracts of various cell lines, using Prohibitin antibody (NBP3-15756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Paraffin: Prohibitin Antibody (0V2R10) [NBP3-15756]

Immunohistochemistry-Paraffin: Prohibitin Antibody (0V2R10) [NBP3-15756]

Immunohistochemistry-Paraffin: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry of paraffin-embedded mouse kidney using Prohibitin antibody (NBP3-15756) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Prohibitin Antibody (0V2R10) [NBP3-15756]

Immunohistochemistry-Paraffin: Prohibitin Antibody (0V2R10) [NBP3-15756]

Immunohistochemistry-Paraffin: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Prohibitin antibody (NBP3-15756) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Prohibitin Antibody (0V2R10)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression.

Alternate Names

PHB

Gene Symbol

PHB1

Additional Prohibitin Products

Product Documents for Prohibitin Antibody (0V2R10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Prohibitin Antibody (0V2R10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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