Prohibitin Antibody (5H7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37563
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey, Primate
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5H7
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified recombinant fragment of human Prohibitin expressed in E. Coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Prohibitin Antibody (5H7) - BSA Free
Western Blot: Prohibitin Antibody (5H7)BSA Free [NBP2-37563]
Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]
Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]
Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.Applications for Prohibitin Antibody (5H7) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Prohibitin
Alternate Names
PHB
Gene Symbol
PHB1
Additional Prohibitin Products
Product Documents for Prohibitin Antibody (5H7) - BSA Free
Product Specific Notices for Prohibitin Antibody (5H7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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