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Prohibitin Antibody (5H7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37563

Novus Biologicals, part of Bio-Techne
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NBP2-37563
NBP2-37563-0.025ml

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5H7

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified recombinant fragment of human Prohibitin expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Prohibitin Antibody (5H7) - BSA Free

Western Blot: Prohibitin Antibody (5H7)BSA Free [NBP2-37563]

Western Blot: Prohibitin Antibody (5H7)BSA Free [NBP2-37563]

Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.

Applications for Prohibitin Antibody (5H7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is a 30 kD member of the band 7 family containing a SPFH domain. Prohibitin is localized the nucleus and mitochondrial inner cell membrane. This protein functions as a negative regulator of cell proliferation associated with senescence and cell death and a transcriptional repressor via recruitment of Brg-1, Brm or N-CoR, or HDAC1. Prohibitin can also function as a membrane-bound chaperone for the assembly of the mitochondrial respiratory chain complex. Prohibitin is induced by metabolic stress caused by imbalance in synthesis of mitochondrial-, nuclear-encoded mitochondrial proteins. Prohibitin can be modified by phosphorylation and ubiquitination. This protein has been shown to interact with Rb, Brg-1, Brm, N-CoR, HDAC1, and PHB2.

Alternate Names

PHB

Gene Symbol

PHB1

Additional Prohibitin Products

Product Documents for Prohibitin Antibody (5H7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Prohibitin Antibody (5H7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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