Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PHB/3194

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Prohibitin Antibodies »

Product Specifications

Immunogen

Recombinant human Prohibitin protein fragment (aa167-261) (exact sequence is proprietary) (Uniprot: P35232)

Localization

Cytoplasmic (Mitochondria)

Marker

Mitochondrial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07841).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Prohibitin Antibody (PHB/3194) - Azide and BSA Free

Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]

Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]

Western Blot: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).
Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]

Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194) labeled with CF488 (Green); RedDot is used to label the nuclei.
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]

Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]

Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).
View 2 more images

Applications for Prohibitin Antibody (PHB/3194) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression.

Alternate Names

PHB

Gene Symbol

PHB1

Additional Prohibitin Products

Product Documents for Prohibitin Antibody (PHB/3194) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Prohibitin Antibody (PHB/3194) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.