Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08752
![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3194-Immunohistochemistry-Paraffin-NBP3-08752-img0002.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
![Protein Array: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3194-Protein-Array-NBP3-08752-img0005.jpg "Protein Array: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
![Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3194-Western-Blot-NBP3-08752-img0004.jpg "Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PHB/3194
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Immunogen
Recombinant human Prohibitin protein fragment (aa167-261) (exact sequence is proprietary) (Uniprot: P35232)
Localization
Cytoplasmic (Mitochondria)
Marker
Mitochondrial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07841).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]
Western Blot: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).![Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3194-Immunocytochemistry-Immunofluorescence-NBP3-08752-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]
Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194) labeled with CF488 (Green); RedDot is used to label the nuclei.![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3194-Immunohistochemistry-Paraffin-NBP3-08752-img0003.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).Applications for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Prohibitin
Alternate Names
PHB
Gene Symbol
PHB1
Additional Prohibitin Products
Product Documents for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Product Specific Notices for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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