Prokineticin R2/PROKR2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45218

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Prokineticin R2/PROKR2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NGNTSFTPNFN corresponding to internal region according to NP_658986.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Prokineticin R2/PROKR2 Antibody

Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218]

Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218]

Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218] - 0.1ug/ml staining of Human Cer(A), Rat Brain (B) and Rat Testis (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Prokineticin R2/PROKR2 Antibody [NBP1-45218]

Immunohistochemistry-Paraffin: Prokineticin R2/PROKR2 Antibody [NBP1-45218]

Immunohistochemistry-Paraffin: Prokineticin R2/PROKR2 Antibody [NBP1-45218] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218]

Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218]

Western Blot: Prokineticin R2/PROKR2 Antibody [NBP1-45218] - (1ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Prokineticin R2/PROKR2 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3-1 ug/ml
Application Notes
Western blot: Approx 45kDa band observed in Human Brain (Cerebellum), Testis and Thyroid Gland and in Rat Brain, Stomach and Testis lysates (calculated MW of 44.0kDa according to Human NP_658986.1 and 47.9kDa according to Rat NP_620434.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Brain (Cortex).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prokineticin R2/PROKR2

Corticotropin Releasing Factor Receptor 2 (CRHR2) expression has been reported in various regions of the brain, as well as in placenta, umbilical vein, heart, epididymis, gastrointestinal tract, adrenal, and skeletal muscle. It shows high-affinity CRF binding and also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. CRCH2 alpha is the dominant isoform and is expressed widely. CRCH2 beta is expressed in the hippocampus, septum, amygdala, heart, and skeletal muscle. CRHR2 gamma is brain-specific. ESTs have been isolated from brain libraries.

Long Name

Prokineticin Receptor 2

Alternate Names

GPR73L1, GPRg2, KAL3, PKR2, ProkineticinR2, PROKR2

Entrez Gene IDs

128674 (Human); 192649 (Rat)

Gene Symbol

PROKR2

UniProt

NP_658986.1 (Human)

Additional Prokineticin R2/PROKR2 Products

Product Documents for Prokineticin R2/PROKR2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Prokineticin R2/PROKR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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