Prolactin Antibody (OTI6B1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02142
![Flow Cytometry: Prolactin Antibody (OTI6B1) [NBP2-02142]](https://resources.bio-techne.com/images/products/Prolactin-Antibody-6B1-Flow-Cytometry-NBP2-02142-img0002.jpg "Flow Cytometry: Prolactin Antibody (OTI6B1) [NBP2-02142]")
![Western Blot: Prolactin Antibody (OTI6B1) [NBP2-02142]](https://resources.bio-techne.com/images/products/Prolactin-Antibody-6B1-Western-Blot-NBP2-02142-img0003.jpg "Western Blot: Prolactin Antibody (OTI6B1) [NBP2-02142]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI6B1
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PRL (NP_000939) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Prolactin Antibody (OTI6B1)
Western Blot: Prolactin Antibody (OTI6B1) [NBP2-02142]
Western Blot: Prolactin Antibody (6B1) [NBP2-02142] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prolactin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prolactin.
Immunocytochemistry/Immunofluorescence: Prolactin Antibody (6B1) [NBP2-02142] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Prolactin.
![Immunohistochemistry-Paraffin: Prolactin Antibody (OTI6B1) [NBP2-02142]](https://resources.bio-techne.com/images/products/Prolactin-Antibody-6B1-Immunohistochemistry-Paraffin-NBP2-02142-img0007.jpg "Immunohistochemistry-Paraffin: Prolactin Antibody (OTI6B1) [NBP2-02142]")
Immunohistochemistry-Paraffin: Prolactin Antibody (OTI6B1) [NBP2-02142]
Immunohistochemistry-Paraffin: Prolactin Antibody (6B1) [NBP2-02142] - Staining of paraffin-embedded Human Ovary tissue using anti-Prolactin mouse monoclonal antibody.![Immunocytochemistry/ Immunofluorescence: Prolactin Antibody (OTI6B1) [NBP2-02142]](https://resources.bio-techne.com/images/products/Prolactin-Antibody-6B1-Immunocytochemistry-Immunofluorescence-NBP2-02142-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Prolactin Antibody (OTI6B1) [NBP2-02142]")
![Immunohistochemistry-Paraffin: Prolactin Antibody (OTI6B1) [NBP2-02142]](https://resources.bio-techne.com/images/products/Prolactin-Antibody-6B1-Immunohistochemistry-Paraffin-NBP2-02142-img0005.jpg "Immunohistochemistry-Paraffin: Prolactin Antibody (OTI6B1) [NBP2-02142]")
![Immunohistochemistry-Paraffin: Prolactin Antibody (OTI6B1) [NBP2-02142]](https://resources.bio-techne.com/images/products/Prolactin-Antibody-6B1-Immunohistochemistry-Paraffin-NBP2-02142-img0006.jpg "Immunohistochemistry-Paraffin: Prolactin Antibody (OTI6B1) [NBP2-02142]")
![Flow Cytometry: Prolactin Antibody (OTI6B1) [NBP2-02142]](https://resources.bio-techne.com/images/products/Prolactin-Antibody-6B1-Flow-Cytometry-NBP2-02142-img0001.jpg "Flow Cytometry: Prolactin Antibody (OTI6B1) [NBP2-02142]")
Applications for Prolactin Antibody (OTI6B1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:50-100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Prolactin
Additional Prolactin Products
Product Documents for Prolactin Antibody (OTI6B1)
Product Specific Notices for Prolactin Antibody (OTI6B1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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