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Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34555

Novus Biologicals, part of Bio-Techne
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NBP2-34555-0.1mg

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # B6.2 + PRLR742

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Semi-Purified human Prolactin R (B6.2); Recombinant human Prolactin R (PRLR742) (Uniprot: P16471)

Localization

Cell surface and cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG1 Kappa

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-34286).

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free

Immunohistochemistry-Paraffin: Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free [NBP2-34555]

Immunohistochemistry-Paraffin: Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free [NBP2-34555]

Immunohistochemistry-Paraffin: Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free [NBP2-34555] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Prolactin Receptor Monoclonal Antibody (B6.2 + PRLR742).

Applications for Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Prolactin R

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Long Name

Prolactin Receptor

Alternate Names

PRLR, ProlactinR

Gene Symbol

PRLR

Additional Prolactin R Products

Product Documents for Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Prolactin R Antibody (B6.2 + PRLR742) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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