Key Product Details

Species Reactivity

Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # T6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Prolactin R Antibodies »

Product Specifications

Immunogen

Purified rat liver PRL receptor.

Specificity

Detects prolactin (PRL) receptor in rat tissues. This does not cross-react with Growth Hormone (GH) receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Prolactin R Antibody (T6) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) - Azide and BSA Free [NB300-531]

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) - Azide and BSA Free [NB300-531]

Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: Prolactin R Antibody (T6) - Azide and BSA Free [NB300-531]

Immunohistochemistry-Paraffin: Prolactin R Antibody (T6) - Azide and BSA Free [NB300-531]

Immunohistochemistry-Paraffin: Prolactin R Antibody (T6) [NB300-531] - Normal biopsies of deparaffinized Rat pituitary gland tissue at a dilution of 1:50.
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) - Azide and BSA Free [NB300-531]

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) - Azide and BSA Free [NB300-531]

Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
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Applications for Prolactin R Antibody (T6) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:25 - 1:200

Immunohistochemistry-Paraffin

1:25 - 1:200

Western Blot

1 ug/ml
Application Notes
WB: Detects an approx. 42 kDa protein representing PRL receptor in NB2 cell lysate.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with 0.1 ml PBS.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prolactin R

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Long Name

Prolactin Receptor

Alternate Names

PRLR, ProlactinR

Entrez Gene IDs

24684 (Rat)

Gene Symbol

PRLR

UniProt

P05710

Additional Prolactin R Products

Product Documents for Prolactin R Antibody (T6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Prolactin R Antibody (T6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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