Prominin 2 Antibody (OTI6B7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47938

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6B7

Concentration

0.53 mg/ml

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Product Summary for Prominin 2 Antibody (OTI6B7)

Immunogen

Full-length protein expressed in 293T cell transfected with human PROM2 expression vector

Specificity

This antibody is specific for Homo sapiens prominin 2 (PROM2), transcript variant 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

91.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Prominin 2 Antibody (OTI6B7)

Western Blot: Prominin 2 Antibody (OTI6B7) [NBP1-47938]

Western Blot: Prominin 2 Antibody (OTI6B7) [NBP1-47938]

Western Blot: Prominin 2 Antibody (6B7) [NBP1-47938] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prominin 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prominin 2.
Immunohistochemistry-Paraffin: Prominin 2 Antibody (6B7) [NBP1-47938] Staining of paraffin-embedded Ovary tissue using anti-Prominin 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Prominin 2 Antibody (OTI6B7) [NBP1-47938]

Immunohistochemistry-Paraffin: Prominin 2 Antibody (OTI6B7) [NBP1-47938]

Immunohistochemistry-Paraffin: Prominin 2 Antibody (6B7) [NBP1-47938] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Prominin 2mouse monoclonal antibody.
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Applications for Prominin 2 Antibody (OTI6B7)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.53 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prominin 2

A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. Several criteria were applied to identify and characterize these new and novel genes. First, a biological signal sequence trap in yeast cells was utilized to identify cDNA clones encoding putative secreted proteins, second strategy utilized various algorithms that recognize features such as the hydrophobic properties of signal sequences to identify putative proteins encoded by expressed sequence tags (ESTs) from human cDNA libraries and finally, ESTs for protein sequence similarity to a set of known receptors and their ligands with the BLAST algorithm. Based on these criteria isolation of full length cDNA clones for each of these genes resulted in identification of more than 1000 novel proteins. One of these new genes is Prominin2. Prominin 2 is a 112 kDa glycoporotein structurally related to Prominin 1 (CD133) although amino acid similarity is not more than 30% but their genomic organization is strikingly similar (1). Like Prominin1, the prominin 2 exhibit similar membrane topology with 5 trans-membrane domains and two large glycosylated extracellular domains. Similar to Prominin1 localization, the Prominin 2 is also associated with membrane protrusions of the epithelial cells from adult kidney, and all along the digestive track and other epithelial tissues. One striking difference between Prominin1 and Prominin 2 expression lies in its conspicuous absence in eye tissue (2). Prominin 2, along with other genes (AP1M2, MAL2, PRSS8 and FLJ20171) expression is differentially expressed in chromophobe renal cell carcinoma compared to benign oncocytoma consistently and this marker can be used to differentiate RCC form benign oncocytoma (3). Prominin 2 is an androgen-responsive genes proapoptotic membrane protein that is expressed in human prostate and human prostate cancer cell lines with highest levels in less aggressive LNCaP cell and low expression in highly aggressive PC3 and DU145 cells suggesting a role of Prominin 2 in down-regulation of aggressiveness of prostate cancer cells (4).

Alternate Names

PROM2, PROML2

Entrez Gene IDs

150696 (Human)

Gene Symbol

PROM2

Additional Prominin 2 Products

Product Documents for Prominin 2 Antibody (OTI6B7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Prominin 2 Antibody (OTI6B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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