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Protease Inhibitor 15 Antibody (3B5)

Novus Biologicals, part of Bio-Techne | Catalog # H00051050-M02

Novus Biologicals, part of Bio-Techne
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H00051050-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PI15 (NP_056970, 18 a.a. ~ 117 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EASTVVLLNSTDSSPPTNNFTDIEAALKAQLDSADIPKARRKRYISQNDMIAILDYHNQVRGKVFPPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYL

Reactivity Notes

Human. Other species not tested.

Specificity

PI15 (3B5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Protease Inhibitor 15 Antibody (3B5)

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody (3B5) [H00051050-M02]

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody (3B5) [H00051050-M02]

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody (3B5) [H00051050-M02] - Analysis of monoclonal antibody to PI15 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Sandwich ELISA: Protease Inhibitor 15 Antibody (3B5) [H00051050-M02] - Detection limit for recombinant GST tagged PI15 is approximately 0.1ng/ml as a capture antibody.

Applications for Protease Inhibitor 15 Antibody (3B5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protease Inhibitor 15

This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system.

Alternate Names

25 kDa trypsin inhibitor, CRISP8, CRISP-8, Cysteine-rich secretory protein 8, P24TI, p25TI, P25TIDKFZp686F0366, peptidase inhibitor 15, PI-15, protease inhibitor 15, sugarCrisp

Entrez Gene IDs

51050 (Human)

Gene Symbol

PI15

OMIM

607076 (Human)

Additional Protease Inhibitor 15 Products

Product Documents for Protease Inhibitor 15 Antibody (3B5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Protease Inhibitor 15 Antibody (3B5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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