Proteasome 19S S3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31530

Novus Biologicals, part of Bio-Techne
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NBP1-31530
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Proteasome 19S S3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 19S S3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 19S S3 Antibody

Immunohistochemistry-Paraffin: Proteasome 19S S3 Antibody [NBP1-31530]

Immunohistochemistry-Paraffin: Proteasome 19S S3 Antibody [NBP1-31530]

Immunohistochemistry-Paraffin: Proteasome 19S S3 Antibody [NBP1-31530] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: Proteasome 19S S3 Antibody [NBP1-31530]

Western Blot: Proteasome 19S S3 Antibody [NBP1-31530]

Western Blot: Proteasome 19S S3 Antibody [NBP1-31530] - Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE, antibody diluted at 1:500.
Proteasome 19S S3 Antibody

Western Blot: Proteasome 19S S3 Antibody [NBP1-31530] -

Western Blot: Proteasome 19S S3 Antibody [NBP1-31530] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Proteasome 19S S3 antibody [N1N3] (NBP1-31530) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Proteasome 19S S3 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Proteasome 19S S3

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Alternate Names

26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, P58, proteasome (prosome, macropain) 26S subunit, non-ATPase, 3, Proteasome subunit p58, Rpn3, S3, 26S proteasome non-ATPase regulatory subunit 3, tissue specific transplantation antigen 2, TSTA2

Gene Symbol

PSMD3

Additional Proteasome 19S S3 Products

Product Documents for Proteasome 19S S3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Proteasome 19S S3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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