Proteasome 19S S3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94013

Novus Biologicals, part of Bio-Techne
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NBP2-94013-0.02ml
NBP2-94013-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Proteasome 19S S3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human Proteasome 19S S3 (O43242).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 19S S3 Antibody - BSA Free

Western Blot: Proteasome 19S S3 AntibodyBSA Free [NBP2-94013]

Western Blot: Proteasome 19S S3 AntibodyBSA Free [NBP2-94013]

Western Blot: Proteasome 19S S3 Antibody [NBP2-94013] - Analysis of extracts of various cell lines, using Proteasome 19S S3 at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Applications for Proteasome 19S S3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 19S S3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. (provided by RefSeq)

Alternate Names

26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, P58, proteasome (prosome, macropain) 26S subunit, non-ATPase, 3, Proteasome subunit p58, Rpn3, S3, 26S proteasome non-ATPase regulatory subunit 3, tissue specific transplantation antigen 2, TSTA2

Gene Symbol

PSMD3

Additional Proteasome 19S S3 Products

Product Documents for Proteasome 19S S3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Proteasome 19S S3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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